Publications

Current Preprints

Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization
Breillat C, Renou E, Darribere M, Rimbault C, Talenton V, Ecoutin A, Daburon S, Poujol C, Choquet D, Mackereth CD, Sainlos M.
bioRxiv 2025.08.24.671450; (2025).
DOI: 10.1101/2025.08.24.671450

A nanobody-gold conjugate for the detection of GFP-tagged synaptic proteins by dual light-electron microscopy
Ducro, C, Lemoigne C, Lecompte Saint-Jean P, Cloatre T, Daburon S, Seiling K, Mohrmann L, Rohlmann A, Missler M, Fronzes R., Choquet D, Sainlos M*, Thoumine O*.
bioRxiv 2025.10.08.681152; (2025).
DOI: 10.1101/2025.10.08.681152

Synchrotron XRF imaging reveals manganese accumulation in the Golgi and post-synapses of neurons and enhanced uptake in astrocytes
Kelkoul I, Kittilukkana A, Huarte L, Castillo-Michel H, Salome M, Roudeau S, Belzanne P, Sainlos M, Pied N, Fernandez-Monreal M, Choquet D, Ortega R, Carmona A.
bioRxiv 2025.09.05.674493; (2025).
DOI: 10.1101/2025.09.05.674493

Dissecting the binding mechanisms of synaptic membrane adhesion complexes using a micropattern based cellular model.
Piette N, Strale P.O., Lagardère M, Saphy C, Reissner C, Munier M, Missler M, Chamma I, Sainlos, M., Thoumine O, Studer V.
bioRxiv 2024.03.17.584836 (2024)
DOI: 10.1101/2024.03.17.584836

Synapse specific and plasticity-regulated AMPAR mobility tunes synaptic integration
Nowacka A, Getz, A.M., Zieger H.L., Bessa-Neto D, Breillat C, Daburon S, Lemoigne C, Marais S, Ducros M, Penn A.C., Sainlos, M., Choquet D.
bioRxiv 2024.03.19.584837 (2024).
DOI: 10.1101/2024.03.19.584837

Published

LRRTM2 controls presynapse nano-organization and AMPA receptor sub-positioning through Neurexin-binding interface
Liouta K, Lubas M, Venugopal V, Chabbert J, Jeannière C, Diaz C, Munier M, Tessier B, Claverol S, Favereaux A, Sainlos M, de Wit J, Letellier M, Thoumine O, Chamma I.
Nat Commun.;15(1):8807 (2024).
DOI: 10.1038/s41467-024-53090-y

Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
Rimbault C, Breillat C, Compans B, Toulmé E, Nunes Vicente F, Fernandez-Monreal M, Mascalchi P, Genuer C, Puente-Muñoz V, Gauthereau I, Hosy E, Claverol S, Giannone G, Chamma I, Mackereth CD, Poujol C, Choquet D, Sainlos M.
eLife, e69620(2024).
DOI: 10.7554/eLife.69620

High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
Getz A.M., Ducros M., Breillat C., Lampin-Saint-Amaux A., Daburon S., François U., Nowacka A., Fernandez-Monreal M., Hosy E., Lanore F., Zieger H.L., Sainlos M., Humeau Y., Choquet D.
Sci. Adv., 8 (30), eabm5298 (2022).
DOI: 10.1126/sciadv.abm5298

High-Resolution Fluorescence Imaging Combined With Computer Simulations to Quantitate Surface Dynamics and Nanoscale Organization of Neuroligin-1 at Synapses
Lagardère, M., Drouet, A., Sainlos, M., Thoumine, O.
Front. Synaptic Neurosci. 14, 835427 (2022)
DOI: 10.3389/fnsyn.2022.835427

MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior.
Toledo, A., Bimbi, G., Letellier, M., Tessier, B., Daburon, S., Faveraux, A., Chamma, I., Vennekens, K. M., Vanderlinden, J., Craig, A. M., Sainlos, M., de Wit, J., Choquet, D., Thoumine, O.
eLife e75233(2022)
DOI: 10.7554/eLife.75233

Role of regulatory C-terminal motifs in synaptic confinement of LRRTM2
Liouta, K., Chabbert, J., Benquet, S., Tessier, B., Studer, V., Sainlos, M., De Wit, J., Thoumine, O., Chamma, I.
Biol Cell 113 (12), 492 (2021)
DOI: 10.1111/boc.202100026

Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration
Mehidi, A., Kage, F., Karatas, Z., Cercy M., Schaks M., Polesskaya, A., Sainlos, M., Gautreau, A., Rossier, O., Rottner, K. & Giannone, G.
Nat. Cell Biol. 23(11), 1148 (2021)
DOI: 10.1038/s41556-021-00786-8

Advanced imaging and labeling methods to decipher brain cell organization and function
Choquet, D., Sainlos, M., Sibarita J.B.
Nat. Rev. Neurosci. 22(4), 237 (2021)
DOI: 10.1038/s41583-021-00441-z

Biophysical mechanisms underlying the membrane interactions of synaptic adhesion molecules.
Chamma, I., Sainlos, M. & Thoumine, O.
Neuropharmacology 169, 107555 (2020)
DOI: 10.1016/j.neuropharm.2019.02.037

TSPAN5 enriched microdomains provide a platform for dendritic spines maturation through Neuroligin-1 clustering
Moretto, E., Longatti, A., Murru, L., Chamma, I., Sessa, A., Zapata, J., Hosy, E., Sainlos, M., Saint-Pol, J., Rubinstein, E., Choquet, D., Broccoli, V., Schiavo, G., Thoumine, O. & Passafaro, M.
Cell. Rep. 29, 1130 (2019).
DOI: 10.1016/j.celrep.2019.09.051

Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules.
Rimbault, C., Maruthi, K., Breillat, C., Genuer, C., Crespillo, S., Puente-Muñoz, V., Chamma, I., Gauthereau, I., Antoine, S., Thibault, C., Wong Jun Tai, F., Dartigues, B., Grillo-Bosch, D., Claverol, S., Poujol, C., Choquet, D., Mackereth, C.D.* & Sainlos, M.*
Nat. Commun. 10 (1), 4521 (2019)
DOI: 10.1038/s41467-019-12528-4

Functional recruitment of dynamin requires multimeric interactions for efficient endocytosis
Rosendale, M.*, Van, T.N.*, Grillo-Bosch, D.*, Sposini, S., Claverie, L., Gauthereau, I., Claverol, S., Choquet, D., Sainlos, M.* & Perrais, D.*
Nat. Commun. 10 (1), 4462 (2019)
DOI: 10.1038/s41467-019-12434-9

Differential Nanoscale Topography and Functional Role of GluN2-NMDA Receptor Subtypes at Glutamatergic Synapses.
Kellermayer, B., Ferreira, J.S., Dupuis, J., Levet, F., Grillo-Bosch, D., Bard, L., Linarès-Loyez, J., Bouchet, D., Choquet, D., Rusakov, D.A., Bon, P., Sibarita, J.-B., Cognet, L., Sainlos, M., Carvalho, A.L. & Groc, L.
Neuron 100 (1), 106 (2018)
DOI: 10.1016/j.neuron.2018.09.012

A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation
Letellier, M., Szíber, Z. Chamma, I., Saphy, C., Papasideri, I., Tessier, B., Sainlos, M., Czöndör, K. & Thoumine, O.
Nat. Commun. 9 (1), 3979 (2018)
DOI: 10.1038/s41467-018-06220-2

Modulation of AMPA receptor surface diffusion restores hippocampal plasticity and memory in Huntington’s disease models.
Zhang, H., Zhang, C., Vincent, J., Zala, D., Benstaali, C., Sainlos, M., Grillo-Bosch, D., Cho, Y., David, D.J., Saudou, F., Humeau, Y. & Choquet, D.
Nat. Commun. 9 (1), 4272 (2018)
DOI: 10.1038/s41467-018-06675-3

Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency.
Haas, K.T., Compans, B., Letellier, M., Bartol, T.M., Grillo-Bosch, D., Sejnowski, T.J., Sainlos, M., Choquet, D., Thoumine, O. & Hosy, E.
eLife 7, e31755 (2018)
DOI: 10.7554/eLife.31755

CaMKII Metaplasticity Drives Aβ Oligomer-Mediated Synaptotoxicity.
Opazo, P., Viana da Silva, S., Carta, M., Breillat, C., Coultrap, S.J., Grillo-Bosch, D., Sainlos, M., Coussen, F., Bayer, K.U., Mulle, C. & Choquet, D.
Cell Rep. 23, 3137-3145 (2018)
DOI: 10.1016/j.celrep.2018.05.036

Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin.
Chamma, I., Rossier, O., Giannone, G. Thoumine, O.* & Sainlos, M.*
Nat. Protoc. 12, 748-763 (2017)
DOI: 10.1038/nprot.2017.010

Nanoscale organization of synaptic adhesion proteins revealed by single-molecule localization microscopy.
Chamma, I., Levet, F., Sibarita, J.B., Sainlos, M. & Thoumine, O.
Neurophotonics 3, 041810 (2016)
DOI: 10.1117/1.NPh.3.4.041810

Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin.
Chamma, I., Letellier, M., Butler, C., Tessier, B., Lim, K.H., Gauthereau, I., Choquet, D., Sibarita, J.B., Park, S., Sainlos, M.* & Thoumine, O.*
Nat. Commun. 7, 10773 (2016)
DOI: 10.1038/ncomms10773

Lengthening of the Stargazin Cytoplasmic Tail Increases Synaptic Transmission by Promoting Interaction to Deeper Domains of PSD-95.
Hafner, A.S., Penn, A.C., Grillo-Bosch, D., Retailleau, N., Poujol, C., Philippat, A., Coussen, F., Sainlos, M. Opazo, P. & Choquet, D.
Neuron 86, 475-489 (2015)
DOI: 10.1016/j.neuron.2015.03.013

Inhibition of PDZ domain-mediated interactions.
Grillo-Bosch, D., Choquet, D. & Sainlos, M.
Drug Discov. Today Technol., 10, e531-e540 (2013)
DOI: 10.1016/j.ddtec.2012.10.003

Neurexin-1β binding to neuroligin-1 triggers the preferential recruitment of PSD-95 versus gephyrin through tyrosine phosphorylation of neuroligin-1.
Giannone, G., Mondin, M., Grillo-Bosch, D., Tessier, B., Saint-Michel, E., Czöndör, K., Sainlos, M., Choquet, D. & Thoumine, O.
Cell Rep., 3, 1996-2007 (2013)
DOI: 10.1016/j.celrep.2013.05.013

Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions.
Sainlos, M., Iskenderian-Epps, W.S., Olivier, N.B., Choquet, D. & Imperiali, B.
J. Am. Chem. Soc., 135, 4580-4583 (2013)
DOI: 10.1021/ja309870q

Paromomycin and neomycin B derived cationic lipids: Synthesis and transfection studies.
Mével, M.*, Sainlos, M.*, Chatin, B., Oudrhiri, N., Hauchecorne, M., Lambert, O., Vigneron, J.-P., Lehn, P., Pitard, B. & Lehn, J.-M.
J. Controlled Release, 158, 461-469 (2012)
DOI: 10.1016/j.jconrel.2011.12.019

Regulation of AMPA receptor surface diffusion by PSD-95 slots.
Opazo, P., Sainlos, M. & Choquet, D.
Curr. Opin. Neurobiol., 22, 453-460 (2012)
DOI: 10.1016/j.conb.2011.10.010

Biomimetic divalent ligands for the acute disruption of synaptic AMPAR stabilization.
Sainlos, M., Tigaret, C., Poujol, C., Olivier, N.B., Bard, L., Breillat, C., Thiolon, K., Choquet, D. & Imperiali, B.
Nat. Chem. Biol., 7, 81-91 (2011)
DOI: 10.1038/nchembio.498

Dynamic and specific interaction between synaptic NR2-NMDA receptor and PDZ proteins.
Bard, L., Sainlos, M., Bouchet, D., Cousins, S., Mikasova, L., Breillat, C., Stephenson, F.A., Imperiali, B., Choquet, D. & Groc, L.
Proc. Natl. Acad. Sci. U. S. A., 107, 19561-19566 (2010)
DOI: 10.1073/pnas.1002690107

Monitoring protein interactions and dynamics with solvatochromic fluorophores.
Loving, G.S., Sainlos, M. & Imperiali, B.
Trends Biotechnol., 28, 73-83 (2010)
DOI: 10.1016/j.tibtech.2009.11.002

A General Screening Strategy for Peptide-Based Fluorogenic Ligands: Probes for Dynamic Studies of PDZ Domain-Mediated Interactions.
Sainlos, M., Iskenderian, W.S. & Imperiali, B.
J. Am. Chem. Soc., 131, 6680-6682 (2009)
DOI: 10.1021/ja900371q

Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells.
Venkatraman, P., Nguyen, T.T., Sainlos, M., Bilsel, O., Chitta, S., Imperiali, B. & Stern, L.J.
Nat. Chem. Biol., 3, 222-228 (2007)
DOI: 10.1038/nchembio868

Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN.
Sainlos, M. & Imperiali, B.
Nat. Protoc., 2, 3219-3225 (2007)
DOI: 10.1038/nprot.2007.444

Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based ‘building block’ approach.
Sainlos, M. & Imperiali, B.
Nat. Protoc., 2, 3210-3218 (2007)
DOI: 10.1038/nprot.2007.443

Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization.
Sainlos, M. & Imperiali, B.
Nat. Protoc., 2, 3201-3209 (2007)
DOI: 10.1038/nprot.2007.442

Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference.
Desigaux, L., Sainlos, M., Lambert, O., Chevre, R., Letrou-Bonneval, E., Vigneron, J.P., Lehn, P., Lehn, J.M. & Pitard, B.
Proc. Natl. Acad. Sci. U. S. A., 104, 16534-16539 (2007)
DOI: 10.1073/pnas.0707431104

Aminoglycoside-quinacridine conjugates: Towards recognition of the P6.1 element of telomerase RNA.
Kaiser, M., Sainlos, M.,, Lehn, J.M., Bombard, S. & Teulade-Fichou, M.P.
ChemBioChem, 7, 321-329 (2006)
DOI: 10.1002/cbic.200500354

Neomycin-capped aromatic platforms: quadruplex DNA recognition and telomerase inhibition.
Kaiser, M., De Cian, A., Sainlos, M., Renner, C., Mergny, J.L. & Telaude-Fichou, M.P.
Org. Biomol. Chem., 4, 1049-1057 (2006)
DOI: 10.1039/b516378a

Kanamycin A-derived cationic lipids as vectors for gene transfection.
Sainlos, M., Hauchecorne, M., Oudrhiri, N., Zertal-Zidani, S., Aissaoui, A., Vigneron, J.P., Lehn, J.M. & Lehn, P.
ChemBioChem, 6, 1023-1033 (2005)
DOI: 10.1002/cbic.200400344

The design of cationic lipids for gene delivery.
Martin, B., Sainlos, M., Aissaoui, A., Oudrhiri, N., Hauchecorne, M., Vigneron, J.P., Lehn, J.M. & Lehn,
Curr. Pharm. Design, 11, 375-394 (2005)
DOI: 10.2174/1381612053382133

Aminoglycoside-derived cationic lipids for gene transfection: Synthesis of kanamycin A derivatives.
Sainlos, M., Belmont, P., Vigneron, J.P., Lehn, P. & Lehn, J.M.
Eur. J. Org. Chem., 2764-2774 (2003)
DOI: 10.1002/ejoc.200300164

Advances in cationic lipid-mediated gene delivery.
Martin, B., Aissaoui, A., Sainlos, M., Oudrhiri, N., Hauchecorne, M., Vigneron, J.P., Lehn, J.M. & Lehn, P.
Gene Ther. Mol. Biol., 7, 273-289 (2003)
DOI: direct link

Progress in gene delivery by cationic lipids: Guanidinium-cholesterol-based systems as an example.
Aissaoui, A., Oudrhiri, N., Petit, L., Hauchecorne, M., Kan, E., Sainlos, M., Julia, S., Navarro, J., Vigneron, J.P., Lehn, J.M. & Lehn, P.
Curr. Drug Targets, 3, 1-16 (2002)
DOI: 10.2174/1389450023348082